Coding

Part:BBa_K1896002:Design

Designed by: Bob Van Hove, Maarten Van Brempt   Group: iGEM16_UGent_Belgium   (2016-10-12)


inaZ (GG safe)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 646
    Illegal NgoMIV site found at 2158
    Illegal NgoMIV site found at 2302
    Illegal NgoMIV site found at 2566
    Illegal NgoMIV site found at 2710
    Illegal NgoMIV site found at 2758
    Illegal NgoMIV site found at 2878
    Illegal AgeI site found at 417
    Illegal AgeI site found at 1822
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 238


Design Notes

The many repeats in this gene seem to make this sequence virtually impossible to synthesize, even after changing the codons used. Over-expression of this part at high levels is toxic.



Source

This part was based on BBa_K584021.


References